• Product NameEndothelin 1 antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationPurified by antigen-affinity chromatography.
• ApplicationsWB IF
• Species ReactivityHu
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide contain a sequence corresponding to a region within amino acids 141 and 203 of Human EDN1
• Target NameEndothelin 1
• Accession No. Swiss-Prot:P05305
  Gene ID:1906
• UniprotP05305
• Gene ID1906;
• Concentration 1mg/ml
• Formulation Supplied in 0.1M Tris-buffered saline with 20% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

• Predicted MW: 24kd
  Western blotting: 1:500-1:3000
  Immunofluorescence: 1:100-1:200
  

The protein encoded by this gene is proteolytically processed to release a secreted peptide termed endothelin 1. This peptide is a potent vasoconstrictor and is produced by vascular endothelial cells. Endothelin 1 also can affect the central nervous system. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

• Sample (30 ug of whole cell lysate)
  A: 293T
  B: Hep G2
  12% SDS PAGE
  Primary antibody diluted at 1: 1000
• Immunofluorescence analysis of methanol-fixed HeLa, using Endothelin 1 antibody at 1: 200 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting