• Product NameDOK2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total DOK2 protein.
• Immunogen Typepeptide
• Immunogen DescriptionSynthetic peptide of human DOK2
• Target NameDOK2
• Alternative Names    p56DOK; p56dok-2
• Accession No. Swiss-Prot#: O60496
  NCBI Gene ID: 9046
• UniprotO60496
• Gene ID9046;
• Calculated MW 45kd
• Concentration 0.4mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1000
  

The protein encoded by this gene is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. This encoded protein binds p120 (RasGAP) from CML cells.

• Gel: 8%SDS-PAGE
  Lysate: 40 μg, Lane: 293T cell lysate,
  Primary antibody:DOK2 antibody at dilution 1/200,
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,
  Exposure time: 90 seconds

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting