• Product NameDUSP3 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification
• ApplicationsIHC WB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total DUSP3 protein.
• Immunogen Typepeptide
• Immunogen DescriptionSynthetic peptide of human DUSP3
• Target NameDUSP3
• Alternative Names    VHR
• Accession No. Swiss-Prot#: P51452
  NCBI Gene ID: 1845
• UniprotP51452
• Gene ID1845;
• Calculated MW 20kd
• Concentration 0.5mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1000
  Immunohistochemistry: 1: 20-100

The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. This gene maps in a region that contains the BRCA1 locus which confers susceptibility to breast and ovarian cancer. Although DUSP3 is expressed in both breast and ovarian tissues, mutation screening in breast cancer pedigrees and in sporadic tumors was negative, leading to the conclusion that this gene is not BRCA1.

• Gel: 12%SDS-PAGE
  Lysate: 40 μg, Lane 1-6: Jurkat£¬Hela£¬K562£¬293T and HepG2 cell£¬Human fetal liver tissue lysates,
  Primary antibody:DUSP3 antibody at dilution 1/250 dilution,
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,
  Exposure time: 30 seconds
• The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using DUSP3 Antibody at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: x200)
• The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using DUSP3 Antibody at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: x200)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting