• Product NameHSPB1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification
• ApplicationsIHC WB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total HSPB1 protein.
• Immunogen Typepeptide
• Immunogen DescriptionSynthetic peptide of human HSPB1
• Target NameHSPB1
• Alternative Names    CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102
• Accession No. Swiss-Prot#: P04792
  NCBI Gene ID: 3315
• UniprotP04792
• Gene ID3315;
• Calculated MW 23kd
• Concentration 0.7mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1000
  Immunohistochemistry: 1: 20-100

The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN).

• Gel: 12%SDS-PAGE
  Lysate: 40 μg, Lane 1-5: 293T£¬Hela£¬K562£¬A431 and HEPG2 cell lysates,
  Primary antibody:HSPB1 antibody at dilution 1/250 dilution,
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,
  Exposure time: 2 seconds
• The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using HSPB1 Antibody at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: x200)
• The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using HSPB1 Antibody at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: x200)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting