• Product NameIQGAP1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification
• ApplicationsIHC WB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total IQGAP1 protein.
• Immunogen Typepeptide
• Immunogen DescriptionSynthetic peptide of human IQGAP1
• Target NameIQGAP1
• Alternative Names    SAR1; p195; HUMORFA01
• Accession No. Swiss-Prot#: P46940
  NCBI Gene ID: 8826
• UniprotP46940
• Gene ID8826;
• Calculated MW 189kd
• Concentration 0.8mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1000
  Immunohistochemistry: 1: 20-100

This gene encodes a member of the IQGAP family. The protein contains four IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. It interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. Expression of the protein is upregulated by gene amplification in two gastric cancer cell lines.

• Gel: 6%SDS-PAGE
  Lysate: 40 μg, Lane: HUVEC cell lysate,
  Primary antibody:IQGAP1 antibody at dilution 1/300,
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,
  Exposure time: 1 minute
• The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using IQGAP1 Antibody at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting