• Product NameDkk-3 antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationPurified by antigen-affinity chromatography.
• ApplicationsWB IF
• Species ReactivityHu
• Immunogen TypeRecombinant protein
• Immunogen DescriptionRecombinant protein fragment contain a sequence corresponding to a region within amino acids 6 and 313 of Dkk-3
• Target NameDkk-3
• Accession No. Swiss-Prot:Q9UBP4
  Gene ID:27122
• UniprotQ9UBP4
• Gene ID27122;
• Concentration 1mg/ml
• Formulation Supplied in 1XPBS, 1%BSA, 20% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

• Predicted MW: 38kd
  Western blotting: 1:500-1:3000
  Immunofluorescence: 1:100-1:200
  

This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq]

• Sample (30 ug of whole cell lysate)
  A: A549
  10% SDS PAGE
  Primary antibody diluted at 1: 1000
• Immunofluorescence analysis of methanol-fixed A549, using DKK3 antibody at 1: 500 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting