• Product NamePI3 Kinase p110 beta antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationPurified by antigen-affinity chromatography.
• ApplicationsWB IF
• Species ReactivityHu
• Immunogen TypeRecombinant protein
• Immunogen DescriptionRecombinant protein fragment contain a sequence corresponding to a region within amino acids 509 and 790 of Human PIK3CB
• Target NamePI3 Kinase p110 beta
• Accession No. Swiss-Prot:P42338
  Gene ID:5291
• UniprotP42338
• Gene ID5291;
• Concentration 1mg/ml
• Formulation Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

• Predicted MW: 123kd
  Western blotting: 1:500-1:3000
  Immunofluorescence: 1:100-1:200
  

Phosphoinositide 3-kinases (PI3Ks) phosphorylate the 3-prime OH position of the inositol ring of inositol lipids. They have been implicated as participants in signaling pathways regulating cell growth by virtue of their activation in response to various mitogenic stimuli. PI3Ks are composed of a 110-kD catalytic subunit, such as PIK3CB, and an 85-kD adaptor subunit (Hu et al., 1993 [PubMed 8246984]).[supplied by OMIM]

• Sample (30 ug of whole cell lysate)
  A: H1299
  7.5% SDS PAGE
  Primary antibody diluted at 1: 1000
• Immunofluorescence analysis of methanol-fixed HeLa, using PI3 kinase p110 beta antibody at 1: 200 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting