• Product NameCUBN Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification
• ApplicationsIHC WB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total CUBN protein.
• Immunogen Typepeptide
• Immunogen DescriptionSynthetic peptide of human CUBN
• Target NameCUBN
• Alternative Names    IFCR; MGA1; gp280
• Accession No. Swiss-Prot#: O60494
  NCBI Gene ID: 8029
• UniprotO60494
• Gene ID8029;
• Calculated MW 399kd
• Concentration 1.4mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1000
  Immunohistochemistry: 1: 30-150

Cubilin (CUBN) acts as a receptor for intrinsic factor-vitamin B12 complexes. The role of receptor is supported by the presence of 27 CUB domains. Cubulin is located within the epithelium of intestine and kidney. Mutations in CUBN may play a role in autosomal recessive megaloblastic anemia.

• Gel: 6%SDS-PAGE
  Lysate: 40 μg, Lane: Human kidney tissue lysate,
  Primary antibody:CUBN antibody at dilution 1/500 dilution,
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,
  Exposure time: 6 minutes
• The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using CUBN Antibody at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting