• Product Namealpha sarcomeric Actin antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationPurified by antigen-affinity chromatography.
• ApplicationsWB IHC
• Species ReactivityHu
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein fragment contain a sequence corresponding to a region within amino acids 99 and 364 of Human ACTC1
• Target Namealpha sarcomeric Actin
• Accession No. Swiss-Prot:P68032
  Gene ID:70
• UniprotP68032
• Gene ID70;
• Concentration 1.56 mg/ml
• Formulation Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

• Predicted MW: 42kd
  Western blotting: 1:500-1:3000
  Immunohistochemistry: 1:100-1:250

Actins are highly conserved proteins that are involved in various types of cell motility. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to four others. The protein encoded by this gene belongs to the actin family which is comprised of three main groups of actin isoforms, alpha, beta, and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Defects in this gene have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC). [provided by RefSeq]

• Sample(30 ug of whole cell lysate)
  A: HeLa S3
  B: MOLT4
  12% SDS PAGE
  Primary antibody diluted at 1: 500
• Immunohistochemical analysis of paraffin-embedded Gastric CA, using alpha Actin (cardiac muscle) antibody at 1: 100 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting