• Product NameCDC25A (Phospho-Tyr59) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of cdc25A only when phosphorylated at tyrosine 59.
• Immunogen TypePeptide
• Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 59 (S-D-Y(p)-E-Q) derived from Human CDC25A.
• Target NameCDC25A
• ModificationPhospho
• Alternative Names    CDC25M3, MPIP1
• Accession No. Swiss-Prot#: P30304
  NCBI Gene ID: 993
• UniprotP30304
• Gene ID993;
• Calculated MW 70kd
• Concentration 1.0mg/mL
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:1000

•  Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDC2 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.

• Western blot analysis of extracts from U87 cells, treated with EGF using CDC25A(Phospho-Tyr59) Antibody #11598. Reference: Nat. Commun. 7:12431 doi: 10.1038/ncomms12431 (2016).
• Immunohistochemical analysis of a variety of human tumor tissue using CDC25A(Phospho-Tyr59) Antibody #11598. Reference: Nat. Commun. 7:12431 doi: 10.1038/ncomms12431 (2016).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting