• Product NameLamin B1 antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationPurified by antigen-affinity chromatography.
• ApplicationsWB IHC
• Species ReactivityHu
• Immunogen TypeRecombinant protein
• Immunogen DescriptionRecombinant protein fragment contain a sequence corresponding to a region within amino acids 289 and 544 of Human LMNB1
• Target NameLamin B1
• Accession No. Swiss-Prot:P20700
  Gene ID:4001
• UniprotP20700
• Gene ID4001;
• Concentration 1mg/ml
• Formulation Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

• Predicted MW: 66kd
  Western blotting: 1:500-1:3000
  Immunohistochemistry: 1:100-1:250
  

The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. [provided by RefSeq]

• Sample(30 ug whole cell lysate)
  A: A431
  B: H1299
  C: HeLa S3
  7.5% SDS PAGE
  Primary antibody diluted at 1: 5000
• Immunohistochemical analysis of paraffin-embedded Colon ca, using Lamin B1 antibody antibody at 1: 250 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting