• Product Namep-Tau antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationPurified by antigen-affinity chromatography.
• ApplicationsWB IHC IF
• Species ReactivityHu
• Immunogen TypePeptide
• Immunogen DescriptionSynthetic peptide contain a sequence corresponding to a region within amino acids 171 and 236 of Human MAPT
• Target Namep-Tau
• Accession No. Swiss-Prot:P10636
  Gene ID:4137
• UniprotP10636
• Gene ID4137;
• Concentration 1mg/ml
• Formulation Supplied in 0.1M Tris-buffered saline with 20% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

• Predicted MW: 79kd
  Western blotting: 1:500-1:3000
  Immunohistochemistry: 1:100-1:500
  Immunofluorescence: 1:100-1:200
  

This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. [provided by RefSeq]

• Sample (30 ug of whole cell lysate)
  A: A431
  7.5% SDS PAGE
  Primary antibody diluted at 1: 1000
• Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using Tau antibody at 1: 500 dilution.
• Immunofluorescence analysis of methanol-fixed A549, using Tau antibody at 1: 500 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting