• Product NameART1 Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse
• Immunogen DescriptionRecombinant fusion protein of human ART1 (NP_004305.2).
• Alternative Names    ART1; ART2; ARTC1; CD296; RT6; ADP-ribosyltransferase 1
• Accession No. Swiss-Prot#:P52961
  NCBI Gene ID:417
• UniprotP52961
• Gene ID417;
• Calculated MW 36kDa
• Formulation Avoid freeze / thaw cycles.|Buffer: PBS with 50% glycerol, pH7.4.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IF 1:50 - 1:200

ADP-ribosyltransferase catalyzes the ADP-ribosylation of arginine residues in proteins. Mono-ADP-ribosylation is a posttranslational modification of proteins that is interfered with by a variety of bacterial toxins including cholera, pertussis, and heat-labile enterotoxins of E. coli. The amino acid sequence consists of predominantly hydrophobic N- and C-terminal regions, which is characteristic of glycosylphosphatidylinositol (GPI)-anchored proteins. This gene was previously designated ART2.

• Western blot analysis of extracts of various cell lines, using ART1 at 1:1000 dilution.
• Immunohistochemistry of paraffin-embedded human lung cancer using ART1 at dilution of 1:100 (40x lens).
• Immunofluorescence analysis of L929 cells using ART1 at dilution of 1:100. Blue: DAPI for nuclear staining.
• Immunofluorescence analysis of U-2 OS cells using ART1 at dilution of 1:100. Blue: DAPI for nuclear staining.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting