• Product NameATF7 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total ATF7 protein.
• Immunogen Typepeptide
• Immunogen DescriptionSynthetic protein corresponding to residues near the N terminal of human ATF7
• Target NameATF7
• Alternative Names    ATFA
• Accession No. Swiss-Prot:P17544
  NCBI Gene ID:11016
  NCBI Protein:BC042363
• UniprotP17544
• Gene ID11016;
• Calculated MW 53 kDa
• Concentration 0.9mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500-1:2000
  Immunohistochemistry: 1: 30-150

Plays important functions in early cell signaling. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AG][AG]-3'), a sequence present in many viral and cellular promoters. Activator of the NF-ELAM1/delta-A site of the E-selectin promoter. Has no intrinsic transcriptional activity, but activates transcription on formation of JUN or FOS heterodimers. Also can bind TRE promoter sequences when heterodimerized with members of the JUN family.

• Gel: 8%SDS-PAGE
  lysate: 40 ¦Ìg, Lane: Hela cell lysate,
  Primary antibody: 46318£¨ATF7 Antibody) at dilution 1/500
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,
  Exposure time: 30seconds
• The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 46318(ATF7 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: x200)
• The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using 46318(ATF7 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: x200)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting