Collagen III (1D4) Mouse mAb - SAB

Catalog No.	Package Size	Price	Quantity
26245-1	50ul	$219.00
26245-2	100ul	$319.00

Datasheet Images Protocol

Description

Product NameCollagen III (1D4) Mouse mAb
Clone No.1D4-5A10-7A4
IsotypeIgG1
PurificationAffinity purified
ApplicationsWB,IHC-P,IF
Species ReactivityHu,Ms,Rt
Immunogen DescriptionSynthetic Peptide of Collagen III
ConjugatesUnconjugated
ModificationUnmodification
Alternative Names CO3A1, Collagen alpha 1(III) chain, Collagen III alpha 1 chain precursor, Collagen type III

Accession No. SwissProtID:P02461
Gene ID:1281
UniprotP02461
Gene ID1281;
Calculated MW 138kDa
Cell Localization Extracellular matrix, Secreted
Tag Info The antibody detects endogenous Collagen III protein.
Formulation PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Storage -20˚C/1 year

Application Details

Images

Western blot analysis of lysates from 1) MCF7 , 2) Hela, 3) 293T cells, （Green） primary antibody was diluted at 1:1000, 4°over night, secondary antibody was diluted at 1:10000, 37° 1hour. （Red） Actin β Polyclonal Antibody was diluted at 1:5000 as loading control, 4° over night,secondary antibody was diluted at 1:10000, 37° 1hour.
Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,Collagen III Monoclonal Antibody(Q76) was diluted at 1:200(4˚C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98˚C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1,Collagen III Monoclonal Antibody(Q76) was diluted at 1:200(4˚C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98˚C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1,Collagen III Monoclonal Antibody(Q76) was diluted at 1:200(4˚C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98˚C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of Mouse-liver tissue. 1,Collagen III Monoclonal Antibody(Q76)(red) was diluted at 1:200(4˚C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Western blot analysis of Hela, diluted at 1:1000.

Published Papers

Please let us know if you have published research using #26245 so that we can cite your reference.

Customer Reviews and Rankings

No comment

Collagen III (1D4) Mouse mAb #26245

Published Papers

Customer Reviews and Rankings

My Review

Application

Species Reactivity