• Product NameLC3B Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionA synthetic peptide of human LC3B (NP_073729.1).
• Alternative Names    LC3B;ATG8F;MAP1LC3B-a;MAP1A/1BLC3;MAP1LC3B;LC3A/LC3B
• Accession No. GeneID:81631
  Swiss Prot:Q9GZQ8
• UniprotQ9GZQ8
• Gene ID81631;
• Calculated MW 14kDa
• SDS-PAGE MW 14kDa/15kDa
• Concentration 1.0mg/ml
• Formulation PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Storage Store at -20˚C. Avoid freeze / thaw cycles.|Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.126.

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IF 1:50 - 1:200

The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.

• Immunofluorescence analysis of LC3B knockout 293T cells treated by chloroquine and wild-type 293T cells ��wild-type 293T cells treated by chloroquine using LC3B Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.
• Immunohistochemistry of paraffin-embedded mouse brain using LC3B antibody at dilution of 1:100 .
• Immunohistochemistry of paraffin-embedded rat brain using LC3B antibody at dilution of 1:100 .
• Western blot analysis of extracts of 293T cells, using LC3B antibody at 1:1000 dilution.293T cells were treated by Chloroquine at 37�� for 20 hours.
• Western blot analysis of extracts of various cell lines, using LC3B antibody at 1:1000 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting