• Product NameHSP90B(Phospho-Ser254) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous level of HSP90B only when phosphorylated at serine 254.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 254 (V-G-S(p)-D-E) derived from Human HSP90B.
• Target NameHSP90B
• ModificationPhospho
• Alternative Names    HS90B; HSP 84; HSP90-beta; HSP90AB1; HSPC2
• Accession No. Swiss-Prot: P08238
  NCBI Protein: NP_031381.2
• UniprotP08238
• Gene ID3326;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 92kd
  Western blotting: 1:500~1:1000

Molecular chaperone. Has ATPase activity.

Zhao R, Houry WA.(2007) Adv Exp Med Biol; 594: 27-36.
Olsen JV, et al. (2006) Cell; 127(3): 635-648
Ogiso H, et al. (2004) Biochemistry; 43(49): 15510-15519

• Western blot analysis of extracts from Hela cells untreated(lane 1) or treated with hot shock(lane 2) using HSP90B(Phospho-Ser254) Antibody #11196.
• Western blot analysis of extracts from Hela cells, treated with calf intestinal phosphatase (CIP), using HSP90B (Phospho-Ser254) Antibody #11196.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting