• Product NameMAP1LC3B Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total MAP1LC3B protein.
• Immunogen Typepeptide
• Immunogen DescriptionSynthetic peptide corresponding to residues near the N terminal of human MAP1LC3B
• Target NameMAP1LC3B
• Alternative Names    LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
• Accession No. Swiss-Prot:Q9GZQ8
  NCBI Gene ID:81631
  NCBI Protein:NP_073729
• UniprotQ9GZQ8
• Gene ID81631;
• Calculated MW 15 kDa
• Concentration 0.9mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1: 20-100

The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.

• Gel: 12%SDS-PAGE
  lysate: 40 ¦Ìg, Lane 1-2: HEPG2 cell and Human fetal brain tissue lysates,
  Primary antibody: 46604£¨MAP1LC3B Antibody) at dilution 1/300
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,
  Exposure time: 5 seconds
• The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 46604(MAP1LC3B Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: x200)
• The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using 46604(MAP1LC3B Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: x200)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting