• Product NameNCBP1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total NCBP1 protein.
• Immunogen Typepeptide
• Immunogen DescriptionSynthetic peptide corresponding to residues near the N terminal of human NCBP1
• Target NameNCBP1
• Alternative Names    NCBP; Sto1; CBP80
• Accession No. Swiss-Prot:Q09161
  NCBI Gene ID:4686
  NCBI Protein:NP_002477
• UniprotQ09161
• Gene ID4686;
• Calculated MW 92 kDa
• Concentration 0.5mg/ml
• Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:200-1:1000
  Immunohistochemistry: 1: 25-100

The product of this gene is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. The encoded protein promotes high-affinity mRNA-cap binding and associates with the CTD of RNA polymerase II. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay.

• Gel: 6%SDS-PAGE
  lysate: 40 ¦Ìg, Lane 1-6: Jurkat£¬NIH/3T3£¬293T£¬Hela£¬HT-29 and HEPG2 cell lysates,
  Primary antibody: 46622£¨NCBP1 Antibody) at dilution 1/200
  Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,
  Exposure time: 40 seconds
• The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using 46622(NCBP1 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200)
• The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 46622(NCBP1 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting