• Product NameSki Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAffinity chromatography purified via peptide column
• ApplicationsELISA WB IF
• Species ReactivityHu
• Immunogen TypePeptide
• Immunogen DescriptionRaised against a 14 amino acid peptide from near the amino terminus of human Ski.
• Target NameSki
• Alternative Names    c-ski, v-ski sarcoma viral oncogene homolog
• Accession No. P12755
• UniprotP12755
• Gene ID6497;
• Concentration 1mg/ml
• Formulation Supplied in PBS containing 0.02% sodium azide.
• Storage Can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

TGF-beta is a ubiquitously expressed cytokine that signals through the Smad protein family to regulate numerous cellular processes such as embryonic development and tumorigenesis. This signaling is negatively regulated by Ski, the mammalian homolog of the transforming protein of an avian retrovirus that induces oncogenic transformation of chicken embryo cells, and the related protein SnoN. Ski functions by binding to the Smad proteins and preventing their phosphorylation, thereby inhibiting their ability to bind DNA and activate the transcription of downstream genes. Ski will also bind to the Smad proteins specific to bone morphogenic proteins (BMPs) and block BMP signaling in mammalian cells.

• Western blot analysis of Ski in human kidney tissue lysate with Ski antibody at (A) 1 and (B) 2 ug/mL.
• Immunofluorescence of Ski in human kidney tissue with Ski antibody at 20 ug/mL.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting