• Product NameNALP1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationImmunoaffinity chromatography purified IgG
• ApplicationsELISA WB ICC
• Species ReactivityHu
• Immunogen TypePeptide
• Immunogen DescriptionRaised against a peptide corresponding to 13 amino acids near the carboxy terminus of human NALP1.
• Target NameNALP1
• Alternative Names    CARD7, DEFCAP, NACHT domain- leucine-rich-repeat and PYD-containing protein 1
• Accession No. Swiss-Prot:Q9C000
  Gene ID:22861
• UniprotQ9C000
• Gene ID22861;
• Concentration 1mg/ml
• Formulation Supplied in PBS containing 0.02% sodium azide.
• Storage Can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

NALP1 belongs to a family of cytoplasmic proteins that have been implicated in cell responses to apoptotic and inflammatory stimuli. It contains a caspase recruitment domain (CARD) in addition to a NACHT domain and leucine rich repeat (LRR). This protein interacts strongly with caspase 2 and weakly with caspase 9, as well as with Apaf-1. Transient overexpression of this gene in cultured cells was sufficient to induce apoptosis. NALP1 mRNA and protein expression levels in myeloid leukemia cells are increased following CREB (cAMP-response element binding protein) activation, suggesting that NALP1 may contribute to modulate the response of these cells to pro-inflammatory stimuli. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for NALP1.

• Western blot analysis of NALP1 in U937 cell lysate with NALP1 antibody at (A) 1, (B) 2 and (C) 4 ug/mL.
• Immunocytochemistry of NALP1 in K562 cells with NALP1 antibody at 10 ug/mL.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting