• Product NameIRE1 Rabbit pAb
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionRecombinant protein of human ERN1.
• Alternative Names    ERN1;IRE1;IRE1P;IRE1a;hIRE1p
• Accession No. Swiss Prot:O75460
  GeneID:2081
• Calculated MW 6kDa/109kDa
• SDS-PAGE MW 130kDa
• Formulation Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Storage Store at -20˚C. Avoid freeze / thaw cycles.

• WB1:500 - 1:2000
  IHC1:50 - 1:200
  IF/ICC1:50 - 1:200

The protein encoded by this gene is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. This protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals.

• Immunohistochemistry of paraffin-embedded human breast cancer using IRE1 Rabbit pAbat dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
• Western blot analysis of extracts of various cell lines, using IRE1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
• Immunofluorescence analysis of U2OS cells using IRE1 Rabbit pAb at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting