• Product NamePan-AKT Rabbit Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionA synthetic peptide of human AKT1/AKT2/AKT3
• Alternative Names    AKT1/AKT2/AKT3
• Accession No. Swiss Prot:P31749/P31751/Q9Y243
  GeneID:207/208/10000
• UniprotP31749/P31751/Q9Y243
• Calculated MW 48kDa/55kDa/51kDa/54kDa
• SDS-PAGE MW 60kDa
• Formulation Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Storage Store at -20˚C. Avoid freeze / thaw cycles.

• WB 1:500 - 1:1000
  IHC 1:50 - 1:100
  IF 1:50 - 1:100

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• Western blot analysis of extracts of various cell lines, using AKT at 1:500 dilution.
• Immunofluorescence analysis of C6 cells using AKT1/AKT2/AKT3 at dilution of 1:100. Blue: DAPI for nuclear staining.
• Immunofluorescence analysis of L929 cells using AKT1/AKT2/AKT3 at dilution of 1:100. Blue: DAPI for nuclear staining.
• Immunofluorescence analysis of U-2 OS cells using AKT1/AKT2/AKT3 at dilution of 1:100. Blue: DAPI for nuclear staining.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting