• Product NameBcl-2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationBcl-2 Antibody is affinity chromatography purified via peptide column.
• ApplicationsELISA WB IHC
• Species ReactivityHu Ms Rt
• Immunogen TypePeptide
• Immunogen DescriptionRaised against a peptide corresponding to 15 amino acids near the N terminus of human Bcl-2.
• Target NameBcl-2
• Accession No. Swiss-Prot:P10415
  Gene ID:596
• UniprotP10415
• Gene ID596;
• Concentration 1mg/ml
• Formulation Supplied in PBS containing 0.02% sodium azide.
• Storage Can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. Bcl-2 is the founding member of a family of over 20 proteins that are critical regulators of apoptosis. These can be divided into two classes: those that inhibit apoptosis and those that promote cell death. Bcl-2 is an inner mitochondrial membrane protein that inhibits apoptosis. It is thought to act by interacting with pro-apoptotic Bcl-2 family members such as Bak and Bad. Overexpression of Bcl-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.

• Immunofluorescence of Bcl 2 in mouse brain tissue with Bcl 2 Antibodyat 20 μg/mL.
• Immunohistochemistry of Bcl 2 in mouse brain tissue with Bcl 2 Antibodyat 5 μg/mL.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting