• Product NameBeta-actin Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAffinity chromatography purified via peptide column
• ApplicationsELISA WB ICC
• Species ReactivityHu Ms Rt
• Immunogen TypePeptide
• Immunogen DescriptionRaised against a 16 amino acid peptide from near the carboxy terminus of human b-actin.
• Target NameBeta-actin
• Alternative Names    Beta actin, b actin
• Accession No. Swiss-Prot:P60709
  Gene ID:60
• UniprotP60709
• Gene ID60;
• Concentration 1mg/ml
• Formulation Supplied in PBS containing 0.02% sodium azide.
• Storage Can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms which have been identified. Like GAPDH, beta-actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.

• Western blot analysis of beta-actin in HeLa cell lysate with beta-actin antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL.
• Immunocytochemistry of beta-actin in HeLa cells with beta-actin antibody at 10ug/mL.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting