• Product NameKeratin 8(Phospho-Ser74) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IF
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of Keratin 8 only when phosphorylated at serine 74.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 74 (L-L-S(p)-P-L) derived from Human Keratin 8 (CK8).
• Target NameKeratin 8
• ModificationPhospho
• Alternative Names    CK 8; CK8; CYK8; Cytokeratin endo A; K8
• Accession No. Swiss-Prot: P05787
  NCBI Protein: NP_002264.1
• UniprotP05787
• Gene ID3856;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 55kd
  Western blotting: 1:500~1:1000
  Immunofluorescence: 1:100~1:200

Keratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.

Nakamichi I, et al. (2002) Mol Biol Cell; 13(10): 3441-3451.
Prochasson P, et al. (2002) Nucleic Acids Res; 30(15): 3312-3322.
Pankov R, et al. (1994) Proc Natl Acad Sci U S A; 91(3): 873-877.
Thorey IS, et al. (1993) Mol Cell Biol; 13(11): 6742-6751

• Western blot analysis of extracts from HepG2 cells untreated or treated with EGF using Keratin 8(Phospho-Ser74) Antibody #11307.
• Immunofluorescence staining of methanol-fixed Hela cells using Keratin 8(Phospho-Ser74) Antibody #11307.
• Western blot analysis of extracts from 293 cells, treated with calf intestinal phosphatase (CIP), using Keratin 8 (Phospho-Ser74) Antibody #11307.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting