• Product NameCD31 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S04-2F3
• IsotypeRabbit IgG
• PurificationAffinity Purified
• ApplicationsWB
• Species ReactivityHuman
• Immunogen DescriptionA synthetic peptide of human CD31
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM
• Accession No. Swiss-Prot:P16284
  GeneID:5175
• UniprotP16284
• Gene ID5175
• Calculated MW Calculated MW: 83 kDa; Observed MW: 130 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/1000;

Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions . Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes .

• Western blot detection of CD31 in Jurkat,3T3 cell lysates using CD31 Rabbit mAb(1:1000 diluted).Predicted band size:83kDa.Observed band size:130kDa.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting