• Product NameProfilin 1 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S06-6H7
• IsotypeRabbit IgG
• PurificationAffinity Purified
• ApplicationsWB IHC
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionA synthetic peptide of human Profilin 1
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    Epididymis tissue protein Li 184a;Profilin I
• Accession No. Swiss-Prot:P07737
  GeneID:5216
• UniprotP07737
• Gene ID5216
• Calculated MW Calculated MW: 15 kDa; Observed MW: 15 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/2000; IHC: 1/50;

Swiss-Prot Acc.P07737.Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Inhibits androgen receptor (AR) and HTT aggregation and binding of G-actin is essential for its inhibition of AR.

• Western blot detection of Profilin 1 in C6,3T3,Hela cell lysates using Profilin 1 Rabbit mAb(1:1000 diluted).Predicted band size:15kDa.Observed band size:15kDa.
• Immunohistochemistry of Profilin 1 in paraffin-embedded Human tonsil using Profilin 1 Rabbit mAb at dilution 1/100

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting