• Product NameCD146 Rabbit mAb
• Clone No.S05-5A2
• IsotypeRabbit IgG
• PurificationAffinity Purified
• ApplicationsWB,IHC,ICC/IF,IP,FC
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionA synthetic peptide of human CD146
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    A32 antigen; CD 146; CD146; CD146 antigen; Cell surface glycoprotein P1H12; Gicerin; MCAM; Melanoma adhesion molecule; Melanoma associated antigen A32;
• Accession No. Swiss-Prot:P43121
  GeneID:4162
• UniprotP43121
• Gene ID4162
• Calculated MW 72kDa
• Concentration 0.3 mg/ml
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB 1:500-1:2000 IHC 1:50-1:200 ICC/IF 1:50-1:200 IP 1:50 FC 1:50

CD146 plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development.

• All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
• Western blot analysis of CD146 expression in (1) A375 cell lysate;(2) HUVEC cell lysate
• Immunohistochemical analysis of paraffin-embedded human uterus, using CD146 Antibody.
• Immunofluorescent analysis of A375 cells, using CD146 Antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting