• Product NamePhospho-BRAF (Thr401) Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S05-9B6
• IsotypeRabbit IgG
• PurificationAffinity Purified
• ApplicationsWB
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionA synthetic phosphopeptide corresponding to residues surrounding Thr401 of human B Raf
• ConjugatesUnconjugated
• ModificationPhosphorylated
• Alternative Names    B-RAF; B-RAF proto-oncogene serine/threonine-protein kinase; BRAF1; RAFB1; RMIL; RMIL serine/threonine-protein kinase; c-RMIL; kinase B-Raf;
• Accession No. Swiss-Prot:P15056
  GeneID:673
• UniprotP15056
• Gene ID673
• Calculated MW Calculated MW: 84 kDa; Observed MW: 84 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/1000;

Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway. May play a role in the postsynaptic responses of hippocampal neurons

• Western blot detection of Phospho-BRAF (Thr401) in K562,C6,3T3,Hela cell lysates using Phospho-BRAF (Thr401) Rabbit mAb(1:1000 diluted).Predicted band size:84kDa.Observed band size:84kDa.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting