• Product NameATG12 Rabbit mAb
• Clone No.S08-2F4
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB,IHC,ICC/IF
• Species ReactivityHuman
• Immunogen DescriptionA synthetic peptide of human Apg12
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    APG12; FBR93; APG12L; HAPG12
• Accession No. Swiss-Prot:O94817
  GeneID:9140
• UniprotO94817
• Gene ID9140
• Calculated MW 20,55kDa
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB 1:1000~1:5000 IHC 1:50~1:200 ICC/IF 1:50~1:200

Swiss-Prot Acc.O94817.Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes.

• All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
• All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
• Immunofluorescent analysis using the Antibody at 1:50 dilution.
• Immunofluorescent analysis using the Antibody at 1:50 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting