• Product Namebeta III Tubulin Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S02-4G7
• IsotypeRabbit IgG
• PurificationAffinity Purified
• ApplicationsWB IHC IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionA synthetic peptide of human beta III Tubulin
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    CDCBM; FEOM3; TUBB4; CDCBM1; CFEOM3; beta-4; CFEOM3A
• Accession No. Swiss-Prot:Q13509
  GeneID:10381
• UniprotQ13509
• Gene ID10381
• Calculated MW Calculated MW: 50 kDa; Observed MW: 50 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/5000; IHC: 1/50; ICC/IF: 1/20;

Swiss-Prot Acc.Q13509.Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance.

• Western blot detection of beta III Tubulin in Rat Brain,Hela cell lysates using beta III Tubulin Rabbit mAb(1:1000 diluted).Predicted band size:50kDa.Observed band size:50kDa.
• Immunofluorescence of beta III Tubulin (green) in Hela using beta III Tubulin Rabbit mAb at dilution 1/5, and DAPI(blue)
• Immunohistochemistry of beta III Tubulin in paraffin-embedded Human Cholangiocarcinoma using beta III Tubulin Rabbit mAb at dilution 1/50

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting