• Product NameE2F1 Rabbit mAb
• Clone No.S03-5G3
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB,IHC,ICC/IF,IP,FC
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionA synthesized peptide derived from human E2F1
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    RBP3; E2F-1; RBAP1; RBBP3
• Accession No. 65kDa
• UniprotQ01094
• Gene ID1869
• Calculated MW Calculated MW: 47 kDa; Observed MW: 70 kDa
• Concentration 0.4 mg/ml
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50 FC 1:50

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses.

• All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
• All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
• Immunofluorescent analysis using the Antibody at 1:50 dilution.
• Immunohistochemical analysis of paraffin-embedded human ovary carcinoma, using E2F1 Antibody.
• Immunofluorescent analysis of HeLa cells, using E2F1 Antibody.
• The SOX4/miR-17-92/RB1 Axis Promotes Prostate Cancer Progression. -Neoplasia

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting