• Product NameE2F4 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S09-7E6
• IsotypeRabbit IgG
• PurificationAffinity Purified
• ApplicationsWB
• Species ReactivityHuman
• Immunogen DescriptionA synthetic peptide of human E2F4
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    E2F-4
• Accession No. Swiss-Prot:Q16254
  GeneID:1874
• UniprotQ16254
• Gene ID1874
• Calculated MW Calculated MW: 44 kDa; Observed MW: 65 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/1000

Swiss-Prot Acc.Q16254.Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F4 binds with high affinity to RBL1 and RBL2. In some instances can also bind RB1. Specifically required for multiciliate cell differentiation: together with MCIDAS and E2F5, binds and activate genes required for centriole biogenesis.

• Western blot detection of E2F4 in K562 cell lysates using E2F4 Rabbit mAb(1:1000 diluted).Predicted band size:44kDa.Observed band size:65kDa.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting