• Product NameEpCAM Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S08-8D1
• IsotypeRabbit IgG
• PurificationAffinity Purified
• ApplicationsWB IHC IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionRecombinant protein of human EpCAM
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1
• Accession No. Swiss-Prot:P16422
  GeneID:4072
• UniprotP16422
• Gene ID4072
• Calculated MW Calculated MW: 35 kDa; Observed MW: 35/39 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/1000; IHC: 1/500; ICC/IF: 1/20;

Swiss-Prot Acc.P16422.May act as a physical homophilic interaction molecule between inTinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.

• Western blot detection of EpCAM in MCF7,HCT116,T47D,Hela cell lysates using EpCAM Rabbit mAb(1:1000 diluted).Predicted band size:35kDa.Observed band size:40kDa.
• Immunofluorescence of EpCAM (green) in MCF-7 using EpCAM antibody at dilution 1/2, and DAPI(blue)
• Immunohistochemistry of EpCAM in paraffin-embedded Human breast cancer tissue using EpCAM Rabbit mAb at dilution 1/50

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting