• Product NameLegumain Rabbit mAb
• Clone No.S06-7G1
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionA synthesized peptide derived from human Legumain
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    AEP; LGMN1; PRSC1
• Accession No. Swiss-Prot:Q99538
  GeneID:5641
• UniprotQ99538
• Gene ID5641
• Calculated MW Calculated MW: 49 kDa; Observed MW: 57,49,36 kDa
• Concentration 0.3 mg/ml
• Formulation Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB 1:1000-1:2000

Swiss-Prot Acc.Q99538.Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. Required for normal lysosomal protein degradation in renal proximal tubules. Required for normal degradation of internalized EGFR. Plays a role in the regulation of cell proliferation via its role in EGFR degradation . May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system.

• All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
• All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
• Western blot analysis of Legumain expression in HeLa cell lysate.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting