• Product NameMiTF Rabbit mAb
• Clone No.S06-4B5
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB;ICC/IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionA synthesized peptide derived from human MITF
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    MI; WS2; CMM8; WS2A; COMMAD; bHLHe32
• Accession No. Swiss-Prot:O75030
  GeneID:4286
• UniprotO75030
• Gene ID4286
• Calculated MW Calculated MW: 59 kDa; Observed MW: 52 kDa
• Concentration 0.8mg/ml
• Formulation Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB 1:1000-1:2000;
  ICC/IF 1:50-1:200

Transcription factor that acts as a master regulator of melanocyte survival and differentiation as well as melanosome biogenesis. Binds to M-boxes (5'-TCATGTG-3') and symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoter of pigmentation genes, such as tyrosinase (TYR).

• All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
• All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
• Immunofluorescent analysis using the Antibody at 1:50 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting