• Product NameMLK3 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S07-2C3
• IsotypeRabbit IgG
• PurificationAffinity Purified
• ApplicationsWB
• Species ReactivityHuman,Mouse
• Immunogen DescriptionA synthetic peptide of human MLK3
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    Mixed lineage kinase 3;Src-homology 3 domain-containing proline-rich kinase
• Accession No. Swiss-Prot:Q16584
  GeneID:4296
• UniprotQ16584
• Gene ID4296
• Calculated MW Calculated MW: 93 kDa; Observed MW: 93 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/2000;

Swiss-Prot Acc.Q16584.Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1) through phosphorylation and activation of MAP2K4/MKK4 and MAP2K7/MKK7. Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle.

• Western blot detection of MLK3 in HepG2��A431 lysates using MLK3 antibody.Predicted band size:93kDa.Observed band size:93kDa.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting