• Product NamePGK1 Rabbit mAb
• Clone No.S04-9H9
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB ICC/IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionA synthetic peptide of human PGK1
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    PGKA; MIG10; HEL-S-68p
• Accession No. Swiss-Prot:P00558
  GeneID:5230
• UniprotP00558
• Gene ID5230
• Calculated MW Calculated MW: 45 kDa; Observed MW: 45 kDa
• Concentration 0.5 mg/ml
• Formulation Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB 1:1000-1:2000
  ICC/IF 1:50-1:200

Catalyzes one of the two ATP producing reactions in the glycolytic pathway via the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein.

• All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
• All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
• Immunocytochemistry analysis of PGK1(green) in Hela using PGK1 antibody,and DAPI(blue).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting