• Product NamePhospho-PKA RII alpha (Ser99) Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S05-7G2
• IsotypeRabbit IgG
• PurificationAffinity Purified
• ApplicationsWB IHC
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionA synthetic phosphopeptide corresponding to residues surrounding Ser99 of human PKA R2
• ConjugatesUnconjugated
• ModificationPhosphorylated
• Alternative Names    PKR2; PRKAR2
• Accession No. Swiss-Prot:P13861
  GeneID:5576
• UniprotP13861
• Gene ID5576
• Calculated MW Calculated MW: 46 kDa; Observed MW: 50 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/1000; IHC: 1/100;

Swiss-Prot Acc.P13861.Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.SitesFeature keyPosition(s)Description

• Western blot detection of Phospho-PKA RIIalpha (Ser99) in Rat Brain,C6 cell lysates using Phospho-PKA RIIalpha (Ser99) Rabbit mAb(1:1000 diluted).Predicted band size:46kDa.Observed band size:50kDa.
• Immunohistochemistry of PKA R2 (Phospho-Ser99) in paraffin-embedded Human tonsil using PKA R2 (Phospho-Ser99) Rabbit mAb at dilution 1/100

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting