• Product NamePP2A alpha/beta Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S04-4G4
• IsotypeRabbit IgG
• PurificationAffinity Purified
• ApplicationsWB IHC IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionA synthetic peptide of human PP2A alpha
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    RP-C; PP2Ac; PP2CA; PP2Calpha
• Accession No. Swiss-Prot:P67775
  GeneID:5515
• UniprotP67775
• Gene ID5515
• Calculated MW Calculated MW: 36 kDa; Observed MW: 36 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/1000-1/2000; IHC: 1/50-1/200; ICC/IF: 1/20;

Swiss-Prot Acc.P67775.PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGO2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I . Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259'.

• Western blot detection of PP2A alpha/beta in Rat Brain,C6,CHO-K1,Hela cell lysates using PP2A alpha/beta Rabbit mAb(1:500 diluted).Predicted band size:36kDa.Observed band size:36kDa.
• Immunocytochemistry of PP2A alpha/beta (green) in hela using PP2A alpha/beta Rabbit mAb at dilution 1/5, and DAPI(blue)
• Immunohistochemistry of PP2A alpha/beta in paraffin-embedded Human lung cancer tissue using PP2A alpha/beta Rabbit mAb at dilution 1/50

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting