• Product NameSPI1 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S07-4D3
• IsotypeRabbit IgG
• PurificationAffinity Purified
• ApplicationsWB
• Species ReactivityHuman
• Immunogen DescriptionA synthetic peptide of human PU.1/Spi1
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    OF; PU.1; SFPI1; SPI-1; SPI-A
• Accession No. Swiss-Prot:P17947
  GeneID:6688
• UniprotP17947
• Gene ID6688
• Calculated MW Calculated MW: 31 kDa; Observed MW: 42 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/1000-1/2000;

Swiss-Prot Acc.P17947.Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing .

• Western blot detection of PU.1/Spi1 in Daudi��mouse liver cell lysates using PU.1/Spi1 Rabbit mAb(1:1000 diluted).Predicted band size:31kDa.Observed band size:42kDa.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting