• Product NameWDR1 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S01-6D6
• IsotypeRabbit IgG
• PurificationAffinity Purified
• ApplicationsWB IHC IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionA synthetic peptide of human WDR1
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    AIP1; NORI-1; HEL-S-52
• Accession No. Swiss-Prot:O75083
  GeneID:9948
• UniprotO75083
• Gene ID9948
• Calculated MW Calculated MW: 66 kDa; Observed MW: 66 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/1000-1/5000; IHC: 1/20-1/50; ICC/IF: 1/20-1/50

Swiss-Prot Acc.O75083.Induces disassembly of actin filaments in conjunction with ADF/cofilin family proteins (PubMed:15629458). Enhances cofilin-mediated actin severing . Involved in cytokinesis. Involved in chemotactic cell migration by restricting lamellipodial membrane protrusions (PubMed:18494608). Involved in myocardium sarcomere organization. Required for cardiomyocyte growth and maintenance . Involved in megakaryocyte maturation and platelet shedding. Required for the establishment of planar cell polarity (PCP) during follicular epithelium development and for cell shape changes during PCP; the function seems to implicate cooperation with CFL1 and/or DSTN/ADF. Involved in the generation/maintenance of cortical tension . Involved in assembly and maintenance of epithelial apical cell junctions and plays a role in the organization of the perijunctional actomyosin belt (PubMed:25792565).

• Western blot detection of WDR1 in Jurkat cell lysates using WDR1 Rabbit mAb(1:500 diluted).Predicted band size:66kDa.Observed band size:66kDa.
• Immunocytochemistry of WDR1 (green) in hela using WDR1 Rabbit mAb at dilution 1/5, and DAPI(blue)
• Immunohistochemistry of WDR1 in paraffin-embedded Human Cholangiocarcinoma using WDR1 Rabbit mAb at dilution 1/50

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting