• Product NameNotch4 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S08-8H5
• IsotypeIgG
• PurificationAffinity Purified
• ApplicationsWB
• Species ReactivityHuman
• Immunogen DescriptionA synthetic peptide of human NOTCH4
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    INT3
• Accession No. Swiss-Prot:Q99466
  GeneID:4855
• UniprotQ99466
• Gene ID4855
• Calculated MW Calculated MW:36 kDa,Observed MW:36 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/2000-1/10000

Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May regulate branching morphogenesis in the developing vascular system (By similarity).

• Western blot detection of NOTCH4 in Hela,U87-MG cell lysates using NOTCH4 Rabbit mAb(1:1000 diluted).Predicted band size:36kDa.Observed band size:36kDa.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting