• Product NamePhospho-E Cadherin (Ser838/Ser840) Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S03-3B3
• IsotypeIgG
• PurificationAffinity Purified
• ApplicationsWB IHC
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionA synthetic phosphopeptide corresponding to residues surrounding Ser838/Ser840 of human E Cadherin
• ConjugatesUnconjugated
• ModificationPhosphorylated
• Alternative Names    UVO; CDHE; ECAD; LCAM; Arc-1; BCDS1; CD324
• Accession No. Swiss-Prot:P12830
  GeneID:999
• UniprotP12830
• Gene ID999
• Calculated MW Calculated MW:98 kDa,Observed MW:135 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/2000-1/10000 IHC: 1/20

Cadherins are calcium-dependent cell adhesion proteins (PubMed:11976333).They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed:11976333).Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.

• Western blot detection of E Cadherin (phospho-Ser838/Ser840) in HepG2,U2OS,U251,Lncap using E Cadherin (phospho-Ser838/Ser840) Rabbit mAb(1:1000 diluted)
• Immunohistochemistry of E Cadherin (phospho-Ser838/Ser840) in paraffin-embedded Human breast cancer tissue using E Cadherin (phospho-Ser838/Ser840) Rabbit mAb at dilution 1/50

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting