• Product NameCofilin Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S06-2C0
• IsotypeIgG
• PurificationAffinity Purified
• ApplicationsWB IHC IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionA synthetic peptide of human Cofilin
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    CFL; cofilin; HEL-S-15
• Accession No. Swiss-Prot:P23528
  GeneID:1072
• UniprotP23528
• Gene ID1072
• Calculated MW Calculated MW:19 kDa,Observed MW:19 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/1000 IHC: 1/50 ICC/IF: 1/50

The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004]

• Western blot detection of Cofilin in A549,MCF-7,293T cell lysates using Cofilin Rabbit mAb(1:1000 diluted).Predicted band size:19kDa.Observed band size:19kDa.
• Immunocytochemistry of Cofilin (green) in hela using Cofilin Rabbit mAb at dilution 1/50, and DAPI(blue)
• Immunohistochemical of Cofilin in Human lung cancer tissue using Cofilin antibody at dilution 1/2

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting