• Product NameInsulin Degrading Enzyme Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S04-4E8
• IsotypeIgG
• PurificationAffinity Purified
• ApplicationsWB IF
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionA synthetic peptide of human Insulin degrading enzyme/IDE
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    INSULYSIN; Insulin degrading enzyme; IDE;
• Accession No. Swiss-Prot:P14735
  GeneID:3416
• UniprotP14735
• Gene ID3416
• Calculated MW Calculated MW:118 kDa,Observed MW:118 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/2000-1/10000 ICC/IF: 1/20

Plays a role in the cellular breakdown of insulin, APP peptides, IAPP peptides, natriuretic peptides, glucagon, bradykinin, kallidin, and other peptides, and thereby plays a role in intercellular peptide signaling

• Western blot detection of Insulin degrading enzyme/IDE in A549,HL-60,U2OS cell lysates using Insulin degrading enzyme/IDE Rabbit mAb(1:1000 diluted).Predicted band size:118kDa.Observed band size:118kDa.
• Immunocytochemistry of Insulin degrading enzyme/IDE (green) in hela using Insulin degrading enzyme/IDE Rabbit mAb at dilution 1/50, and DAPI(blue)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting