• Product NameRad51 Rabbit mAb
• Clone No.S04-3C6
• IsotypeIgG
• PurificationAffinity-chromatography
• ApplicationsWB;IHC;ICC/IF;IP;FC
• Species ReactivityHuman;Mouse;Rat
• Immunogen DescriptionA synthesized peptide derived from human RAD51
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    RECA; BRCC5; FANCR; MRMV2; HRAD51; RAD51A; HsRad51; HsT16930
• Accession No. Swiss-Prot:Q06609
  GeneID:5888
• UniprotQ06609
• Gene ID5888
• Calculated MW Calculated MW:37 kDa,Observed MW:37 kDa
• Concentration 2mg/ml
• Formulation Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB 1:1000-1:5000
  IHC 1:100-1:200
  ICC/IF 1:50-1:200
  IP 1:20-1:50
  FC 1:20-1:100

Rad51 participates in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Underwinds duplex DNA and forms helical nucleoprotein filaments.

• All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
• Western blot analysis of Rad51 in (1)HEK293 cell lysate; (2)K562 cell lysate.
• Immunohistochemical analysis of paraffin-embedded mouse kidney, using Rad51 Antibody.
• Immunofluorescent analysis using the Antibody at 1:50 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting