• Product NameNEDD8 Activating Enzyme E1C Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S05-6C8
• IsotypeIgG
• PurificationAffinity Purified
• ApplicationsWB
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionA synthetic peptide of human UBA3
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    NAE2; UBE1C; hUBA3
• Accession No. Swiss-Prot:Q8TBC4
  GeneID:9039
• UniprotQ8TBC4
• Gene ID9039
• Calculated MW Calculated MW:52 kDa,Observed MW:52 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/2000-1/10000

Catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression.

• Western blot detection of UBA3 in Hela,A549,HL-60 cell lysates using UBA3 Rabbit mAb(1:1000 diluted).Predicted band size:52kDa.Observed band size:52kDa.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting