• Product NameHuman IgG4 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S08-6H4
• IsotypeIgG
• PurificationAffinity Purified
• ApplicationsWB IHC
• Species ReactivityHuman
• Immunogen DescriptionA synthesized peptide derived from human IgG4
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    Ig gamma 4 chain C region; IGHG4; IGG4;
• Accession No. Swiss-Prot:P01861
  GeneID:
• UniprotP01861
• Calculated MW 36kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/1000-1/2000 IHC: 1/500-1/1000

Swiss-Prot Acc.P01861.IgG4 antibodies will dominate the IgG response in schistosomiasis, lymphatic filariasis, and in patients after allergen immunotherapy. Unlike the other IgG subclasses, IgG4 does not activate complement. A combined IgA-IgG4 deficiency has been associated with recurrent pyogenic infections.

• Western blot detection of IgG4 in HL-60,U2OS cell lysates using IgG4 Rabbit mAb(1:1000 diluted).Observed band size:36kDa.
• Immunohistochemical of IgG4 in Human tonsil tissue using IgG4 antibody at dilution 1/50

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting